Figure 5
From: Photosynthetic production of enantioselective biocatalysts
Functional expression of esterase ST0071 in Synechocystis sp. 6803. (a) SDS-PAGE of a functional expression of the sfGFP-fusion protein of esterase ST0071 (64 kD) in Synechocystis sp. PCC 6803 (b) Selective activity staining using α-naphthylacetate and Fast Red [29]. Cultures were grown in BG-11 media [28] in shake flasks and tubes at 30°C under a light strength of 300 μmol photons m-2 s-1 under an atmosphere containing 5% of CO2.