Table 1 Strains and plasmids used in this study

Strain

AL17

Same as JCL260

[19]

AL1963

AL17 with ascB::Kan^r

This Study

MG1655

F⁻ lambda⁻ ilvG- rfb-50 rph-1

ATCC

XL-1Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacI ^q Z∆M15 Tn10 (Tet^r)]

Agilent Technologies

BL21(DE3)

F^- ompT hsdSB(rB ⁻ , mB ⁻) gal dcm (DE3)

NEB

Plasmids

pSA69

p15A ori; Kan^R; P _LlacO₁:alsS-ilvCD

[19]

pAL377

ColE1 ori; Amp^R; P _LlacO₁

[29]

pAL450

pSC101* ori; Cm^R; P _LtetO₁:osmY-bglC

[29]

pAL451

p15A ori; Cm^R; P _LtetO₁:osmY-bglC

[29]

pAL590

p15A ori; Kan^R P _LlacO₁:alsS-ilvCD P _LtetO₁:osmY-bglC

This Study

pAL536

p15A ori; Cm^R; P _LtetO₁

[29]

pAL603

ColE1 ori; Amp^R; P _LlacO₁:kivd-adhA, P _LlacO₁:alsS-ilvCD

[29]

pAL856

p15A ori; Cm^R; P _LtetO₁ :osmY-bglC

This Study

pAL869

p15A ori; Cm^R; P _LtetO₁ :osmY-CBAP

This Study

pAL952

ColE1 ori; Amp^R; P _T7: His6x-ascB

This Study

pAL959

pBBR32 ori; Amp^R; P _LlacO₁: ascB

This Study

pAL982

pSC101* ori; Amp^R; P _LlacO₁:ascB

This Study