Figure 6

Final yield (mg/L) of ω-hydroxy fatty acids by FoCYP539A7 and FoCYP655C2 with the homologous reductase ( FoCPR ) in the biotransformation carried out at pH 5.5 and pH 7.0. Data were plotted from the 50 hr biotransformation reaction samples. ΔPox1 mutant S. cerevisiae cells harboring the CYP539A7-FoCPR and CYP655C2-FoCPR reconstituted systems were induced with 4% galactose, 2 mM 5-ALA and 500 μM of substrates: caprylic acid (C8), capric acid (C10) and lauric acid (C12) were added and cultured at pH 5.5 and pH 7.0. Samples collected at 10 hr intervals were extracted, trimethylsilyl derivatized and analyzed by GC.