Table 3 List of additives commonly used to promote refolding of solubilized proteins
From: Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process
Common additives used in refolding buffer | References |
|---|---|
Chaotropes | Â |
   Urea | [19] |
   Guanidine hydrochloride | [74] |
Amino acids | Â |
   Glycine | [100] |
   Arginine | |
   Proline | |
Sugars and polyhydric alcohols | Â |
   Sucrose | [104] |
   Polyethylene glycol | |
   Sorbitol | [110] |
   Glycerol | |
Others | Â |
   Sulfobetaines | |
   Substituted pyridines and pyrolles |  |
   Acid substituted aminocyclohexanes |  |