Figure 6

Reduced AATase expression increases specific activity in whole cell lysate assays. A,B) Atf1-S.c (A) and Atf-S.l (B) activity in E. coli whole cell lysates normalized to the expression level from induction with 1 μM IPTG. Error bars represent standard deviation (n = 3). C,D) Western blot analysis of Atf1-S.c (C) and Atf-S.l (D) expression levels in E. coli with inductions of 1, 10, and 100 μM IPTG.