Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates

Table 2 Experimental lipid yields and productivity of different R. toruloides strains grown on different substrates

Carbon source	Lipid productivity	Condition	Lipid yield ^* (g/g substrate)	Lipid yield (C-mol.C-mol ⁻¹ substrate)	Reference
Carbon source	(g/L/h)	Condition	Lipid yield ^* (g/g substrate)	Lipid yield (C-mol.C-mol ⁻¹ substrate)	Reference
glucose	0.15	Batch- N limiting, pO2 controlled	0.15	0.29	This work
glycerol	0.06	Batch- N limiting, pO2 controlled	0.24	0.47
glucose + glycerol	0.12	Batch- N limiting, pO2 controlled	0.17	0.33
glucose	0.07	Batch- N limiting, pO2 un-controlled	0.12	0.23
glycerol	0.06	Batch- N limiting, pO2 un-controlled	0.27	0.53
glucose + glycerol	0.07	Batch- N limiting, pO2 un-controlled	0.2	0.38
Glucose + glycerol + xylose	0.08	Batch- N limiting, pO2 controlled	0.22	0.42
(10:20:30)	0.08	Batch- N limiting, pO2 controlled	0.22	0.42
Biomass hydrolysate + glycerol	0.09	Batch- N limiting, pO2 controlled	0.17	--
(glucose- xylose -glycerol =10:20:30)	0.09	Batch- N limiting, pO2 controlled	0.17	--
glucose	-	Batch, phosphorous limiting	0.21	--	[17]
glucose	-	Continuous- nitrogen limiting	0.19	--	[38]

TAG molecule with 51 carbons and a molecular weight of 801.27 g/mol are used to calculate the yields in C-mol/C-mol.
*lipid yield was calculated from the slope between lipid concentration against consumed sugar(s).

ISSN: 1475-2859