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Figure 5 | Microbial Cell Factories

Figure 5

From: Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system

Figure 5

SDS-PAGE analysis of cell free extract of crude L. plantarum overexpressing β-galactosidase from L. reuteri from the cultivations without pH control (A) and with pH control at pH 6.5 (B). L. plantarum WCFS1 harbouring pEH9R was cultivated in 400-ml laboratory fermentors at 37°C using MRS medium with 20 g/l glucose, and samples were taken at different time points. The arrows indicate the LacL and LacM subunits of the recombinant β-galactosidase. M denotes the Precision protein ladder (Biorad, CA, USA).

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Microbial Cell Factories

ISSN: 1475-2859

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