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Figure 1 | Microbial Cell Factories

Figure 1

From: Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system

Figure 1

Time course of the cultivations of L. plantarum overexpressing β-galactosidase from L. reuteri without pH control. L. plantarum WCFS1 harbouring the pEH9R plasmid was grown in 50-ml cultures using MRS medium with 20 g/l glucose, at 37°C. Recombinant protein expression was induced by the addition of varying amounts of the inducing pheromone IP (ng/ml fermentation broth; see inset) at different phases of the cultivation, i.e., different OD600 values: immediately after inoculation of the culture (A), at OD600 of 0.4-0.5 (B), or at OD600 of 1.5 (C). All data points represent the average value from 2 independent experiments.

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Microbial Cell Factories

ISSN: 1475-2859

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