Figure 6

Effect of whole-cell catalyst addition, with and without additional substrate, on 15β-hydroxycyproterone acetate production. Reactions were performed in shake flasks with 1 mM cyproterone acetate and an initial catalyst concentration of 50 g/L WCW. After 4 h, additional 150 g/L WCW was added to the reaction represented with △, both catalyst and substrate (1 mM) were added to the reaction represented with □ and buffer was added to the control (○) to compensate for the change in volume.