Figure 12From: Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst Comparison of 1 mM cyproterone acetate transformation in bioreactor and shake flask. The reactions were carried out using the same batch of cells, in a resting cell format (100 g/L WCW), and the substrate was added dissolved in DMSO. The initial rate of the reaction performed in the bioreactor (△) showed a higher initial rate compared to the reaction performed in shake flasks (□), although the reaction in the shake flask resulted in a higher final conversion.Back to article page