Figure 2

HPLC chromatogram of the CYP11B1 dependent conversion of 11-deoxycortisol to cortisol by recombinant E. coli . Chromatograms were obtained from extracted samples of E. coli cultures after 0 h (dotted line) and 24 h (solid line) of incubation after transfer into potassium phosphate buffer and addition of 1.2 mM substrate. Substance peaks obtained with an acetonitrile/water gradient represent cortisol with a retention time of 9.0 min and 11-deoxycortisol with a retention time of 14.3 min.