Table 1 Comparative the solubilized protein of EGFP and MMP-12_CAT from inclusion bodies in different concentration of urea by two different methods
Composition of buffer | Protein Conc. (ug/ml) | |||
|---|---|---|---|---|
EGFP | MMP-12_CAT | |||
Urea-denatured | Freeze-thawing | Urea-denatured | Freeze-thawing | |
PBS, pHÂ 8, 0Â M urea | 115 | 241 | 58 | 202 |
PBS, pHÂ 8, 1Â M urea | 116 | 987 | 101 | 1399 |
PBS, pHÂ 8, 2Â M urea | 164 | 1167 | 109 | 1594 |
PBS, pHÂ 8, 3Â M urea | 252 | 1274 | 145 | 1623 |
PBS, pHÂ 8, 4Â M urea | 432 | 1314 | 505 | 1601 |
PBS, pHÂ 8, 5Â M urea | 1335 | 1289 | 1837 | 1580 |
PBS, pHÂ 8, 6Â M urea | 1371 | 1331 | 1819 | 1292 |
PBS, pHÂ 8, 7Â M urea | 1262 | 1187 | 1755 | 1861 |
PBS, pHÂ 8, 8Â M urea | 1246 | 1181 | 1681 | 1523 |