Figure 8From: A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form Fluorescence and CD spectra of solubilized EGFP from inclusion bodies. (A) Fluorescence spectra of solubilized EGFP from freeze-thawing method (2Â M urea) and traditional urea-denatured method (8Â M urea). (B) Far UV CD spectra of solubilized EGFP by freeze-thawing method.Back to article page