Figure 2

SDS-PAGE analysis of EGFP and MMP-12_CAT over-expression in E.coli as inclusion bodies. E. coli BL21 (DE3) cells harbouring expression vector of EGFP and MMP-12 catalytic domain, respectively, were induced with IPTG at 37°C for 4 hours. Equivalent numbers of uninduced (U₁ and U₂) and induced cells (I₁ and I₂) are shown. Equivalent proportions of the soluble fractions (S₁ and S₂) and insoluble inclusion bodies (P₁ and P₂) are shown. The number 1 represents E.coli cells harbouring MMP-12_CAT expression vector and number 2 represents E.coli cells harbouring EGFP expression vector. MMP-12_CAT runs as the band of 22 kDa and EGFP as the band of 27 kDa in accordance with their expected size. Molecular weight markers (M) are shown and their sizes in kilodaltons are indicated on the left.