Figure 5From: A strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation Construction and verification of precorrin-3A synthesis pathway. A) Schematic diagram of precorrin-3A pathway. B) Construction of E. coli strains BL21DE3 (p15ABCDAI). C): The color change of the culture supernatant of E. coli strains BL21DE3 (p15ABCDAI). These strains were cultured in LB medium plus with 1Â g/L 5-aminolevulinic acid.Back to article page