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Table 1 Comparison of concentrations of the 4KB-PE40 IT, PE or the scFv alone inhibiting protein synthesis by 50% of control values (IC 50 )

From: Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

 

Daudi

Ramos

HSB-2

H9

 

IC 50

IT

7 nM

4 nM

>300 nM

>300 nM

PE

200-300 nM

>750 nM

60 nM

>750 nM

scFV

>3200 nM

>3200 nM

>3200 nM

>3200 nM

  1. CD22+ cell lines Daudi and Ramos or CD22− lines HSB-2 and H9 were exposed for 48 h to the 4KB scFv-derived immunotoxin (IT) or to native PE exotoxin A (PE) or 4KB antibody fragment alone (scFv) and cytotoxicity was evaluated by protein synthesis inhibition assay as described in the Methods section.
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Microbial Cell Factories

ISSN: 1475-2859

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