Figure 2From: Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems Constructs for the expression of toxin-based fusions in E. coli. Schematic representation of 4KBscFv (A), PE (B and C) and saporin (D)-derived constructs. Restriction enzyme sites used for the cloning strategy are also shown (for details, see text under Methods section). Sequence of the 218 linker (218 L) in fuchsia color is: GSTSGSGKPGSGEGSTKG (amino acid one letter code).Back to article page