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Figure 11 | Microbial Cell Factories

Figure 11

From: Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

Figure 11

Cleavage pattern assessment of secreted PE. (A) Western blot analysis of native PE fragments derived from PE cleaved under different conditions. CTR (control): native PE incubated with PBS; αA BMMY: native PE incubated with BMMY after 48 h of induction of the GS115 mock transformant pPICZαA (αA) clone; αA BMMY PMSF: as αA BMMY but PE was incubated in addition + 1 mM PMSF BMMY: induction medium only. (B) In silico study of identifiable furin-like cleavage sites in the native PE sequence.

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Microbial Cell Factories

ISSN: 1475-2859

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