Table 2 Primers used in the bioengineering of bacteria to produce higher alcohols
Number | Primers name | Primer 5′-3′ |
|---|---|---|
A1 | LEU2-S | ACATGCATGCGGATGTCTGCCCCTAAGAAGAT |
| Â | LEU2-AS | ACGCGTCGACTTAAGCAAGGATTTTCTTAACTTC |
A2 | ILV2-S | ACGCGTCGACAAGGAGCCAGATGATCAGACAATCTACGCT |
| Â | ILV2-AS | CGCGGATCCTCAGTGCTTACCGCCTGTAC |
A3 | ILV5-S | CGCGGATCCAAGGAGGCCTCATGTTGAGAACTCAAGCCGC |
| Â | ILV5-AS | GCGAGCTCTTATTGGTTTTCTGGTCTCAACTT |
B1 | ILV2-S | AAAACTGCAGATGATCAGACAATCTACGCT |
| Â | ILV2-AS | ACGCGTCGACTCAGTGCTTACCGCCTGTAC |
B2 | ILV5-S | ACAGGTCGACAAGGAGGTCATGTTGAGAACTCAAGCCGC |
| Â | ILV5-AS | CGCGGATCCTTATTGGTTTTCTGGTCTCAA |
B3 | ILV3-S | CGCGGATCCAAGGAGCTGCATGGGCTTGTTAACGAAAGT |
| Â | ILV3-AS | GCGAGCTCTCAAGCATCTAAAACACAACC |
C1 | BAT2-S | CGGCCTGCAGGATGACCTTGGCACCCCTAGA |
| Â | BAT2-AS | CGCGGATCCTCAGTTCAAATCAGTAACAA |
D2 | LEU1-S | TCCCCCCGGGAAGGAGACTAATGGTTTACACTCCATCCAA |
| Â | LEU1-AS | GCGAGCTCCTACCAATCCTGGTGGACTTT |
C2 | leu1-2as | CGCGGATCCAAAGGAGGCCGCATGGTTTACACTCCATCCAAGG |
| Â | leu1-2s | TCCCCCCGGGCTACCAATCCTGGTGGACTTT |
C3 | leu2-2s | TCCCCCCGGGAAAGGAGGCCGCATGTCTGCCCCTAAGAAGATC |
| Â | leu2-2as | GCGAGCTCTTAAGCAAGGATTTTCTTAAC |
D1 | leu4-2as | CGGCCTGCAGGATGGTTAAAGAGAGTATTAT |
| Â | leu4-2s | TCCCCCCGGGTTATGCAGAGCCAGATGCCG |
D2 | LEU1-S | TCCCCCCGGGAAGGAGACTAATGGTTTACACTCCATCCAA |
| Â | LEU1-AS | GCGAGCTCCTACCAATCCTGGTGGACTTT |
F1 | leu1-2Â s | CGGCCTGCAGGATGGTTTACACTCCATCCAA |
| Â | leu1-2as | CGCGGATCCCTACCAATCCTGGTGGACTT |
G1 | pBL1-as | TCCCCGCGGATTCGGGGTCGTTCACTGGT |
| Â | pBL1-s | CCATCGATAACAACAAGACCCATCATAG |
K1 | Kivd-s | AAAACTGCAGATGTATACAGTAGGAGATTACCT |
| Â | Kivd-as | GCTCTAGATTATGATTTATTTTGTTCAGC |
H1 | ADH2-s | GCTCTAGAAGGAAACTCAATGTCTATTCCAGAAACTCAA |
| Â | ADH2-as | CGGGGTACCTTATTTAGAAGTGTCAACAACG |