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Figure 4 | Microbial Cell Factories

Figure 4

From: In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation

Figure 4

Effect of N-acetylglucosamine on glycoconjugate synthesis. A) Comparison of specific EPA-2a yield in the absence (−) or presence (+N-Ac) of N-acetylglucosamine (4 g/L) during simultaneous induction of PglB and EPA in shake flasks. B) EPA-2a expression in the presence of increasing amounts of N-acetylglucosamine (0 g/L – 10 g/L) in 96-deep well plates. Values were divided by the corresponding final OD600 before normalization to exclude that the beneficial effect of N-acetylglucosamine is only due to increase of biomass formation. E. coli cells were incubated at 30°C, and PglB and EPA were induced simultaneously for 24 h by addition of 1 mM IPTG and 2 g/L arabinose, respectively. C). The final OD600 values in the presence of increasing amounts of N-acetylglucosamine (0 g/L – 10 g/L) in 96-deep well plates. Error bars represent the standard deviations of three biological replicates.

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Microbial Cell Factories

ISSN: 1475-2859

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