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Figure 2 | Microbial Cell Factories

Figure 2

From: In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation

Figure 2

Time course of in vivo glycosylation. EPA-2a producing E. coli cells were cultivated at 30°C in shake flasks (A) or in a 1 L-bioreactor (B and C). Samples were taken periodically after induction with 1 mM IPTG and 2 g/L arabinose followed by preparation of OD600-normalized periplasmic extracts and analysis by sandwich ELISA (A and B) or Western blot with anti-EPA antibody (C).

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Microbial Cell Factories

ISSN: 1475-2859

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