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Table 3 Ratio of insoluble to soluble protein fraction

From: Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

Time Wild type enzyme S56P S167P K170E
(His-LOV-BSLA)
0.0 h 0.0 0.0 0.0 0.0
3.0 h 0.0 0.0 0.0 0.0
4.5 h 0.0 0.0 0.0 0.0
6.0 h 1.0 1.0 0.8 1.0
7.5 h 3.6 1.9 2.1 2.3
9.0 h 4.4 4.5 3.1 3.8
10.5 h 4.1 4.0 4.1 4.1
13.0 h 4.4 4.2 4.2 4.1
14.5 h 3.1 3.0 3.9 3.8
18.0 h 3.8 3.4 3.3 3.5
  1. Ratio of insoluble to soluble protein fraction determined from TotalLab TL100 software analyzing the relative peak area of the respective protein bands. Ratios are calculated for four E. coli BL21(DE3) clones presented in Figure 7.