Table 3 Ratio of insoluble to soluble protein fraction
Time | Wild type enzyme | S56P | S167P | K170E |
|---|---|---|---|---|
(His-LOV-BSLA) | ||||
0.0Â h | 0.0 | 0.0 | 0.0 | 0.0 |
3.0Â h | 0.0 | 0.0 | 0.0 | 0.0 |
4.5Â h | 0.0 | 0.0 | 0.0 | 0.0 |
6.0Â h | 1.0 | 1.0 | 0.8 | 1.0 |
7.5Â h | 3.6 | 1.9 | 2.1 | 2.3 |
9.0Â h | 4.4 | 4.5 | 3.1 | 3.8 |
10.5Â h | 4.1 | 4.0 | 4.1 | 4.1 |
13.0Â h | 4.4 | 4.2 | 4.2 | 4.1 |
14.5Â h | 3.1 | 3.0 | 3.9 | 3.8 |
18.0Â h | 3.8 | 3.4 | 3.3 | 3.5 |