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Figure 4 | Microbial Cell Factories

Figure 4

From: Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

Figure 4

Quantitative classification of 15 E. coli BL21(DE3) clones into Type A and Type B according to their respiration behavior. Comparison of the respiration behavior (oxygen transfer rate) as function of time of Type A and Type B clones calculating the ratio between integral X (from first OTR peak to minimum) and integral Y (from minimum to second peak). Integral X represents the phase of protein formation; integral Y represents the second growth phase on residual glycerol. According to Table 2, the ratio X/Y is in the range of 1.4-2.4 for the nine clones specified in Figure 3 belonging to Type A, and in a range of 0.4-0.6 for the six clones belonging to Type B.

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Microbial Cell Factories

ISSN: 1475-2859

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