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Table 4 Strain characteristic physiological parameters determined for the benchmark strain cultivated in hemin-supplemented media

From: Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Hemin [μM]

Δtime adapt [h]

Y X/MeOH [C-mol/C-mol]

Y CO2/MeOH [C-mol/C-mol]

C-balance

q MeOH [mmol/g/h]

q p [U/g/h]

r p [U/L/h]

0.1

6.5

0.04

0.89

0.93

0.69

1.10

34.3

1.0

6.4

0.04

0.93

0.97

0.71

1.26

36.8

10.0

6.5

0.03

0.89

0.96

0.70

2.35

73.2

  1. Δtimeadapt, time for adaptation of the culture to methanol; YX/MeOH, YCO2/MeOH, yields of biomass or CO2 per C-mol of substrate methanol; C-balance, sum of YX/MeOH and YCO2/MeOH which ideally should result in 1.0; qMeOH, average specific uptake rate of methanol during consecutive methanol pulses; qp, specific HRP productivity; rp, volumetric HRP productivity.
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Microbial Cell Factories

ISSN: 1475-2859

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