Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Table 3 Strain specific physiological parameters of the benchmark strain with and without ALA supplementation

ALA [1 mM]	Δtime _adapt [h]	Y _X/MeOH [C-mol/C-mol]	Y _CO2/MeOH [C-mol/C-mol]	C-balance	q _MeOH [mmol/g/h]	q _p [U/g/h]	r _p [U/L/h]
+	8.1	0.03	0.95	0.98	0.73	1.08	36.7
-	8.2	0.04	0.94	0.98	0.76	1.09	37.2

Δtime_adapt, time for adaptation of the culture to methanol; Y_X/MeOH, Y_CO2/MeOH, yields of biomass or CO₂ per C-mol of substrate methanol; C-balance, sum of Y_X/MeOH and Y_CO2/MeOH which ideally should result in 1.0; q_MeOH, average specific uptake rate of methanol during consecutive methanol pulses; q_p, specific HRP productivity; r_p, volumetric HRP productivity calculated from the point of induction until the end of cultivation.

ISSN: 1475-2859