Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Table 1 In silico identification of HEM homologs in P. pastoris

*S. cerevisiae*	*P. pastoris*			Sequence identity [%]
Gene annotation	Gene annotation	Chromosome	COG annotation	Sequence identity [%]
HEM1	HEM1	II	5-aminolevulinate synthase	67.7
HEM2	HEM2	IV	delta-aminolevulinic acid dehydratase	75.3
HEM3	HEM3	I	porphobilinogen deaminase	54.0
HEM4	HEM4	II	uroporphyrinogen-III synthase	42.3
HEM12	n/a	III	uroporphyrinogen decarboxylase	73.2
HEM13	HEM13	III	coproporphyrinogen III oxidase	65.5
HEM14	n/a	IV	protoporphyrinogen oxidase	33.7
HEM15	n/a	III	protoheme ferro-lyase (ferrochelatase)	61.2

Genes HEM12, HEM14 and HEM15 were not annotated (n/a) for P. pastoris CBS 7435. Their chromosomal position in the genome, eukaryotic cluster of orthologous groups (COG; [19]) annotations and identities of the encoded amino acid sequences from S. cerevisiae and P. pastoris are shown.

ISSN: 1475-2859