Figure 7

Peptide purification after enzymatic cleavage through selective precipitation. Selective precipitation was performed by adding ethanol up to 50% (v/v). A) Analysis of supernatant showed 1 major peak corresponding to SA2 B) SEC analysis of cleaved SUMO-SA2 before (solid line) and after (dashed line) selective precipitation. C) ESI-MS mass spectrum of the SA2 peptide in the supernatant after ethanol precipitation.