Identification and characterization of the nitrate assimilation genes in the isolate of Streptomyces griseorubens JSD-1

Table 4 Primers used for quantitative RT-PCR

Gene	Primer (5′-3′)	Target location	PCR size (bp)
16S rRNA	F: CGTATTCACCGCAGCAATGC	94-179	86
16S rRNA	R: GCGAGGTGGAGCGAATCTCA	94-179	86
NarB	F: GACCTCTACGGCTGGCAGGC	259-402	144
NarB	R: GCGGTCGTCAAGCAGGGT	259-402	144
NirD	F: GCGTGGTCGTGCTGTGCGA	101-207	107
NirD	R: CGCCAGGTCCGTCAGCGACA	101-207	107
GlnA	F: GCTGAGCCTGATGGAACGCA	1227-1322	96
GlnA	R: GCCTCCCACTCCTGCTTCT	1227-1322	96
GltG	F: CGGTGTGCCGTTCTGTCA	150-269	120
GltG	R: AAGTTGTTCGTGGCGTGC	150-269	120
GdhH	F: AAACTGCCGACTGGGACC	82-189	108
	R: GCGGTCGGTGAGGTCTTC

ISSN: 1475-2859