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Table 3 PHB accumulation of the strains in xylose or glycerol

From: Engineering of Serine-Deamination pathway, Entner-Doudoroff pathway and pyruvate dehydrogenase complex to improve poly(3-hydroxybutyrate) production in Escherichia coli

Carbon source

Strains a

CDW (g/L) b

PHB (g/L) b

PHB content (%CDW) b

Xylose

JM109(pBHR68)

3.76±0.09

1.79±0.05

47.5±0.86

Xylose

SD06(pBHR68)

5.72±0.05

3.90±0.06

68.1±1.00

Xylose

SD07(pBHR68)

5.70±0.13

3.92±0.04

68.8±1.06

Glycerol

JM109(pBHR68)

3.38±0.08

1.37±0.06

38.4±1.23

Glycerol

SD06(pBHR68)

3.91±0.05

2.15±0.10

54.9±1.72

Glycerol

SD07(pBHR68)

3.79±0.07

2.12±0.08

56.0±1.03

  1. aBacteria were cultured for 48 h in mineral salt medium supplemented with 1 g/L yeast exact and 20 g/L xylose/glycerol at 37°C at a rotation rate of 220 rpm under aerobic conditions.
  2. bData were expressed as average values and standard deviations (SD) of three parallel studies.
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Microbial Cell Factories

ISSN: 1475-2859

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