Table 1 Oligonucleotides used
From: An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis
Primer | Function | Sequence (5′ to 3′) |
|---|---|---|
aguAq F | aguA expression analysis (F) | TTGTGCCGCTTCATAAAATGG |
aguAq R | aguA expression analysis (R) | CACCTGGTGAAGTGGCTTGTATT |
aguRq F | aguR expression analysis (F) | CGGGTTCATCTGATTGATTTTCTTC |
aguRq R | aguR expression analysis (R) | CGTGATTTTCCTCTGTCGGTTCTT |
recA F | recA internal control (F) | CAAGGCTTAGAGATTGCCGATG |
recA R | recA internal control (R) | ACGAGGAACTAACGCAGCAAC |
EFV583-tufF | tuf internal control (F) | CAGGACATGCGGACTACGTTAA |
EFV583-tufR | tuf internal control (R) | TAGGACCATCAGCAGCAGAAAC |
AguR-EntBglII | Cloning P aguR -AguR-P aguB cassette (F) | CCCCAGATCT TTAAAAAGAAACAAGGTGGTGGCCG |
AguR EntNco | Cloning P aguR -AguR-P aguB cassette (R) | CCCCATGG TGTGTTCCTCCTAAAAGTTGTTTTTG |
Expvfor1 | Insertion of His-tag (F) | CACACACACCCATGGCTAATCGACTGCAGGAAAATTTATACTTCCAAGGTC |
Expvrev1 | Insertion of His-tag (R) | CTATCAATCAAAGCAACACGTG |
GfF1 | Cloning of GFP (F) | CACACACACCCATGGAATTCAGTAAGGGAGAAGAACTTTTC |
GfR1 | Cloning of GFP (R) | CACACACACCTGCAG ACTAGTTTTGTAGAGCTCATCCATGC |