Table 1 Effect of growth temperature on protein solubility and conformational quality
From: Expanding the recombinant protein quality in Lactococcus lactis
Fluorescence (A.U./ml · OD) % | Specific fluorescence (A.U./μg) % | ||
|---|---|---|---|
30°C | Soluble fraction | 18,32 | 59,40 |
Insoluble fraction | 81,68 | 40,60 | |
25°C | Soluble fraction | 15,19 | 26,26 |
Insoluble fraction | 84,81 | 73,74 | |
16°C | Soluble fraction | 0,02 | 68,09 |
Insoluble fraction | 99,98 | 31,91 |