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Figure 5 | Microbial Cell Factories

Figure 5

From: High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

Figure 5

High-temperature cultivation of an rhIL-10 expression strain induces ER-phagy. (A) The rhIL-10 expression strain (clone `H') was methanol-induced for 24 hours. Total RNA was extracted, and the mRNA levels of primary autophagy genes were examined by qRT-PCR. The statistical results are based on three independent experiments and represent the mean ± SD (***P <0.001). (B) A typical transmission electron micrograph of a yeast cell at 20°C. (C) Typical transmission electron micrographs of yeast cells at 30°C. ER-containing autophagosomes (ERAs) are labeled with white arrows. Vacuoles, nuclei, endoplasmic reticula, Golgi and mitochondria are labeled as `V,' `N,' `ER,' `G' and `M,' respectively. (D) The normalized length of the cortical ER was measured using transmission electron micrographs. Data represent the mean ± SD (***P <0.001).

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Microbial Cell Factories

ISSN: 1475-2859

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