Figure 3

Display of multiple antigens fused to one Hbp carrier in E. coli . (A-B) Display of antigens fused to the passenger of the non-cleaved HbpD. E. coli MC1061 cells expressing either Hbp(Δβ-cleav), HbpD-Ag85B_[C+N], HbpD-Ag85B_[C+N]-ESAT6 or HbpD-Ag85B_[C+N]-ESAT6-Rv2660c were analyzed as described in the legend to Figure 2 by Coomassie staining (A) and immunoblotting (B). Non-cleaved Hbp species (*) are indicated. (C) Cells described under A and cells carrying the empty vector (EV) pEH3 were fixed and analyzed by immuno-EM using the indicated antibodies as described before [15]. Scale bar: 100 nm.