Figure 5

MEL production from Avicel® (a) and wheat straw (WS) (b) by fed-batch SSF (at 28°C) with (pre-)hydrolysis (at 50°C) using P. antarctica PYCC 5048 ^T and low enzyme loading with prior dialysis. D-Glucose (squares), D-xylose (circles) and MEL (triangles) concentrations. Feeding strategy 1 (continuous lines): initial 40 g/l glucan content (4.0% w/v Avicel^® or 6.6% w/v wheat straw) for pre-hydrolysis (48 h) and further 20 g/l glucan feeding (2.0% w/v Avicel^® or 3.3% w/v wheat straw) at day 0 and day 4. Feeding strategy 2 (dashed lines): initial 60 g/l glucan content (6.0% w/v Avicel^® or 9.9% w/v WS) for pre-hydrolysis (48 h) and further 20 g/l glucan feeding (2.0% w/v Avicel^® or 3.3% w/v WS) at day 4.