Figure 4
From: Smart sustainable bottle (SSB) system for E. coli based recombinant protein production
Production of GST-GFP and TRX-hLIF in a conventional 2-L bioreactor and the SSB system with “booster” amino acids addition. Autoinduction cultivations for the production of GST-GFP (A1-A5) and TRX-hLIF (B1-B5) were carried out in 2-L bioreactor (A1 and B1), the SSB system (A2 and B2), and the SSB system with “booster” amino acids addition (A3/4 and B3/4). The carbon dioxide (CTR, black line) and oxygen transfer rates (OTR, gray line), and the dissolved oxygen concentration (light gray line) are shown. “booster” amino acids additions are indicated by arrows in A4 and B4. GST-GFP (A5) and TRX-hLIF (B5) production in a 2-L bioreactor (lanes 1-3), the SSB system (lanes 4-6), and the SSB system with “booster” amino acids addition (lanes 7-9) were analyzed by SDS-PAGE. W: whole cell protein, S: soluble part, and I: insoluble part of whole cell protein.