Figure 10
Experimental design studies for the identification of mixtures of [1- 13 C]/[U- 12 C]/[U- 13 C]-glucose isotopomers for “general” optimization or for the best resolution (“partial” optimization) of θ 31 , θ 14 , θ 22 fluxes (I) , results of these flux estimations (II) obtained through 13 C-MFA of the simulated SLEs ( A – F ) or PLEs ( G ) with the mentioned mixed tracers: A − SLE, 0/50/50, %% (partially optimized for θ 12 ); B − SLE, 10/44/46, %% (partially optimized for θ 22 ); C − SLE, 13/0/87, %% (partially optimized for θ 31 ); D − SLE, 66/0/34, %% (partially optimized for θ 14 ); E − SLE, 89/0/11, %% (partially optimized for θ 10 ); F −78/0/22, %% (generally optimized); G − PLE consisting of five LEs using the same tracers as in A – E for each experiment (red 68% CI boxes); (III) the normalized flux precision function values for the confidence level of γ = 0.95 computed for these (A – G) experiments. Green 68% CI boxes in (II) indicate flux estimation resulted from 13C-MFA of SLE optimized for best resolution of this flux. The results of the experimental design studies computed by OpenFLUX2 were visualized in the form of a ternary plot generated by commercially available OriginPro 9.1 (Originlab Corp., Northampton, MA, USA) software.