Using ionic liquids in whole-cell biocatalysis for the nucleoside acylation

Table 1 Acylation of ara-C with VL by P. fluorescens in IL-containing systems ^a

Entry	Solvents^b,c	V₀(mmol/L · h)	Y(%)	5′-regioselectivity(%)
1	THF	2.42	4.6	>99.0
2	[EMI][BF₄]/THF	0.05	2.4	>99.0
3	[BMI][BF₄]/THF	0.03	3.2	>99.0
4	[HMI][BF₄]/THF	0.23	4.8	>99.0
5	[OMI][BF₄]/THF	0.25	7.5	>99.0
6	[BMI][PF₆]/THF	2.34	81.1	>99.0
7	[BMI][Br]/THF	0	0	0
8	[BMI][Cl]/THF	0	0	0
9	[BMI][TF₂N]/THF	3.15	70.4	>99.0

^aThe reaction conditions: 50 mg/mL biomass, 20 mmol/L ara-C, 500 mmol/L VL, 40 μL water, 1 mL solvents, 40°C, 200 rpm and 144 h. ^bIL content, 10% v/v; ^cPyridine (10%, v/v) was added to the reaction media as an assistant solubilizer for dissolving the substrate.

ISSN: 1475-2859