Table 1 Comparison of human insulin production systems[70]

Destination of product

Cytoplasm

Secreted

Secreted

Secreted

Biomass cell dry weight (g/l)

80, in bioreactor with fed-batch culture

1.2, in shake flask with batch culture

5, in shake flask with batch culture

59, in bioreactor with fed-batch culture

Typical spec. growth rate (1/h)

0.08- 0.12

not specified

< 0.33

<0.03

Typical spec. production rate (mg/gh)

14.2

3.4

0.21

0.375

Product concentration (g/L)

4.34

0.009

0.075

3.075

Productivity (mg/l h)

1,085

4.01

1.04

17

Reference

[71]

[72]

[19]

[73]