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Table 1 Calculated ATP and reductant yields, and measured metabolites for growth on different substrates in bioreactors

From: Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum

 

Import to Acetyl-CoA

 

Acid Production

    
 

ATP

NADH

Fdred*

AC:BU‡

ATP

NAD+

NetATP

H2:CO2 ¥

H2

NetR.E.#

Glucose

2

2

2

0.82(±0.01)

1.45

1.10

3.45

1.15(±0.01)

2.30

0.60

Gluconate

0.667

1

2

3.89(±0.05)

1.80

0.41

2.47

1.09(±0.01)

2.18

0.41

Galacturonate

0.667

0

2

36.79(±5.44)

1.97

0.05

2.64

0.83(±0.03)

1.66

0.29

  1. Measured values are bold and represent two biological replicates.
  2. Theoretical calculations are italicized.
  3. *Reduced ferredoxin.
  4. ‡Acetate:Butyrate.
  5. ¥Measured during maximal gas production.
  6. #Net reducing equivalents (NADH+Fdred-NAD+-H2) available for biosynthetic reactions.
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Microbial Cell Factories

ISSN: 1475-2859

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