Table 1 Analytic data of N-terminally truncated ST6Gal-I constructs
Construct | Intensity of variant in ESMS [%] | MS analysis | Truncation | IgG1 | Asialofetuin | |
|---|---|---|---|---|---|---|
G2 + 1SA [%] | G2 + 2SA [%] | Fetuin [RFU/ μg] | ||||
Δ48ST6Gal-I | 100 | NYLS… | Δ114 | 7 | 0 | n.d. |
Δ62ST6Gal-I* | 75 | NYLS… | Δ114 | 22 | 0 | 70 |
25 | WKNYLS… | Δ112 | ||||
Δ62ST6Gal-I | 30 | LQKIWKNYLS… | Δ108 | 29 | 0 | 208 |
70 | NYLS… | Δ114 | ||||
Δ62ST6Gal-I | 50 | LQKIWKNYLS… | Δ108 | 51 | 0 | 186 |
20 | NYLS… | Δ114 | ||||
15 | IWKNYLS… | Δ111 | ||||
10 | WKNYLS… | Δ112 | ||||
Δ62ST6Gal-I | 100 | LQKIWKNYLS… | Δ108 | 63 | 34 | 664 |
Δ62ST6Gal-I | 100 | LQKIWKNYLS… | Δ108 | 82 | 15 | 689 |
Δ89ST6Gal-I | - | - | - | n.d. | n.d. | n.d. |