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Figure 3 | Microbial Cell Factories

Figure 3

From: High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

Figure 3

Expression analysis of human FLAG-Δ108ST6Gal-I in P.pastoris . Analysis by A) SDS-PAGE and Western Blot using B) anti-α2,6ST6 and C) anti-FLAG for detection. Lane 1: Novex sharp protein standard; lane 2–6: fermentation supernatant (300 μL TCA precipitated) after 24, 48, 72, 96 and 120 h of induction in shake flasks without addition of protease inhibitor.

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Microbial Cell Factories

ISSN: 1475-2859

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