Figure 2

NoV VLPs purification. Time course for VLP production. Coomasie brilliant blue stained SDS-PAGE of production and purification of the VP1 protein. A) Chromatogram of VLP purification. Solid line represents absorbance at 280 nm and dashed line percentage of NaCl in the buffer. The arrow indicates the elution peak of the VLPs. B) Aliquots from ion exchange purification. The numbers correspond to the aliquot number during the NaCl gradient. After purification, pools 4 to 7 where dialyzed against BA 50 mM and lyophilized. Part of the lyophilized protein was resuspended in PBS. C) and D) SDS-PAGE and Western Blot using anti-NoV from this pool are shown, respectively. In each gel the molecular weights are represented with the correspondent value. Arrows indicate the mobility of the VP1 band in the SDS-PAGE.