Table 1 Primer pairs used in PCR in this study
Primer | Nucleotide sequence (5′→3′) | Product size (bp) |
|---|---|---|
#1: Asi9H1-S | CGNTAYGCNCGNGGNTGG | 728 |
#2: Asi9H2-A | NACIGGRTARTGRCARTT | |
#3: Asi9H3-S | TGYCCNTTYCAYGAYTGG | 532 |
#2: Asi9H2-A | NACIGGRTARTGRCARTT | |
#4: Asi9H7-S | GGNAGYGGIATHACNCC | 530 |
#5: Asi9H5-A | CCYTCYCKRCAIGARTANGG | |
#6: UpKshB-F | GGTAAGCTTACCGGTCGGCGAGCTCCTTGAACTCGTC | 5212 |
#7: DnKshB-R | TCCGAATTCGGATCGACGCCCTCGGGCGGGACAC | |
#8: Inv-F | TGTCGGTACCCACTCGGCACCCGGTCCCAGGAAGG | 6669 |
#9: Inv-R | AGAATCTAGATTCTCGAGCGCGCGCACGAATCCTC | |
#10: Kan-F | ATCTTCTAGAAGCTAGCTTCACGCTGCCGCA | 1159 |
#11: Kan-R | ATTGAGGTACCCTCAGAAGAACTCGTCAAGAAGG | |
#10: Kan-F | ATCTTCTAGAAGCTAGCTTCACGCTGCCGCA | 2786 |
#12: Down-R | TCTCGTGAAGGAATTCGCGAACG | |
#13: KshB-F | GAGCGGATCCGCTCCCGATCAGCGGGAGCCGGAA | 2812 |
#12: Down-R | TCTCGTGAAGGAATTCGCGAACG | |
#13: KshB-F | GAGCGGATCCGCTCCCGATCAGCGGGAGCCGGAA | 1182 |
#14: KshB-R | CTGAAAGCTTTCAGAACTCGATCTTGAGGTGATCGGT |