Figure 5

Nattokinase production in supernatants of wild-type and mutant cultures. (a) Nattokinase activity in the supernatants of wild-type and mutant cultures, Wild-type (wt) (black columns), LM2531 (mut) (gray columns). (b) SDS-PAGE analysis. 1. B. subtilis 168/pBE2 supernatant, 2. LM2531/pBE2 supernatant as control, 3. B. subtilis 168/pBNA supernatant at 6 h, 4. LM2531/pBNA supernatant at 6 h, 5. B. subtilis 168/pBNA supernatant at 12 h, 6. LM2531/pBNA supernatant at 24 h, 7. B. subtilis 168/pBNA supernatant at 24 h, 8. LM2531/pBNA supernatant at 24 h. The arrow indicates the position corresponding to mature nattokinase (29 kDa). (c): fibrinolytic activity assay. 1. B. subtilis 168/pBE2 supernatant, 2. LM2531/pBE2 supernatant, 3. B. subtilis 168/pBNA cell lysate, 4. LM2531/pBNA cell lysate, 5. B. subtilis 168/pBNA supernatant, 6. LM2531/pBNA supernatant.