Figure 4

Intervention strategies suggested by CASOP and cut set analysis in the RN model. The red crosses represent suggested knockout targets. Cut set analysis reveals that deletion of all these targets blocks all CEF/AEF pathways and leads thus to a fixed ATP/NADPH ratio of 1.28 generated by photosynthesis via the remaining LEF (thick arrows). Ethanol synthesis becomes then mandatory to readjust the ATP/NADPH balance for biomass synthesis. The mechanisms (a)-(d) show overexpression (or flux enhancement) targets suggested by CASOP all of which will enforce an increased turnover (wasting) of ATP. For abbreviations and general explanations see Figure 1.