Recombinant GFP is produced in the pre-induction phases of 1 L glucose-grown P. pastoris bioreactor cultivations. GFP yield (mg L-1; green squares) was measured in the culture supernatant in all phases of duplicate 1 L glucose-grown P. pastoris cultivations. The residual glucose concentration (mM; white inverted triangles) was at its highest during phase I, eventually dropping to zero in phase II. The residual methanol (g L-1; black squares) ranged from 2.83 to 8.69 g L-1. The DCW (g L-1; triangles) for this cultivation reached a maximum of 30 g L-1. All measurements were made in triplicate. The error bars represent the SEM.