Table 2 List of strains and plasmids engineered and/or used in this study
From: Rational and combinatorial approaches to engineering styrene production by Saccharomyces cerevisiae
Strain | Genotype | Source |
|---|---|---|
E. coli | Â | Â |
NEB10β | araD139 ∆(ara,leu)7697 fhuA lacX74 galK16 galE15 mcrA f80d(lacZ∆M15)recA1 relA1 endA1 nupG rpsL rph spoT1∆(mrr-hsdRMS-mcrBC) | New England Biolabs |
One Shot ccdB Survival 2 T1 | F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697galU galK rpsL (StrR) endA1 nupG fhuA::IS2 | Life Technologies |
S. cerevisiae | Â | Â |
BY4741 | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 | Thermo Scientific |
BY4741ΔFDC1 (YDR539W) | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 fdc1Δ | Thermo Scientific |
22A | phenylalanine overproducer evolved from BY4741 | This Study |
22A75D | phenylalanine overproducer evolved from 22A | This Study |
BY4741-PAL | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 p425GPDPAL | This Study |
BY4741ΔFDC1-PAL | MATa his3Δ0 leu2Δ0 met15Δ0 ura3Δ0 fdc1Δ p425GPDPAL | This Study |
22A75D-PAL | 22A75D harboring p425GPDPAL | This Study |
22A75D10-PAL | 22A75D aro10Δ harboring p425GPDPAL | This Study |
22A75D104-PAL | 22A75D aro10Δ::aro4 K229L harboring p425GPDPAL | This Study |
Plasmids | Â | Â |
pTrc99A | P trc , pBR322 ori, lacI q , Amp R | Prather Lab, MIT |
pFA6-KanMX | KanR2, pBR322 ori, Amp R | AddGene |
pDONR221 | attP1-ccdB-Cm R-attP2 cassette, pUC ori, Kan R | Life Technologies |
pDONR-PAL | PAL2 from A. thaliana inserted into pDONR221 | This study |
p425GPD | P GPD , attR1-ccdB-Cm R-attR2 cassette, pBR322 ori, LEU2, Amp R | AddGene |
p425GPDPAL | PAL2 from A. thaliana inserted into p425GPD | This study |
pACYCDuet-1 | P T7lac , p15A ori, lacI q , Cm R | Novagen |
pACYC-ARO4K229L-KanMX | pACYCDuet-1 with the integration cassette aro4 K229L-KanMX | This Study |
pUN15-PAL2 | Clone U12256 containing AT3G53260 (PAL2) from A. thaliana | ABRC |