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Figure 2 | Microbial Cell Factories

Figure 2

From: Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation

Figure 2

Scheme of the plasmid pGLG61/XYL3/PDC1. Expression cassettes GAPp-XYL3-AOXt and GAPp-PDC1-AOXt are shown as gray and white boxes, respectively. The geneticin resistance gene (APH), linked to the impaired constitutive gene promoter, encoding glyceraldehyde-3-phosphate dehydrogenase (HpGAPpr) and H. polymorpha LEU2 gene are shown as black and light-gray boxes, respectively. The telomeric region (TEL188) as an autonomously replicating sequence is designated with the hatched lines. Origin of replication (ORI) and ampicillin resistance gene (bla) – arrows. Restriction sites: RI, EcoRI; Xb, XbaI; ScII, SacII; Nr, NarI; Nd, NdeI; N, NotI; P, PstI.

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Microbial Cell Factories

ISSN: 1475-2859

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