Table 2 Strains and plasmids used in this study
From: Exploiting Issatchenkia orientalis SD108 for succinic acid production
Strains or plasmids | Characteristics | Reference or source |
|---|---|---|
Strains | Â | Â |
I. orientalis | Â | Â |
ATCC 24210 | Wild type | NRRL |
SD108 | Wild type | This study |
Io−ura3 | SD108/ura3− | This study |
Io−ura3−+−ura3 | Io−ura3/ura3−::ura3 | This study |
Io−ura3−+−SA | Io−ura3/ura3−::ura3-succinic acid biosynthetic pathway | This study |
S. cerevisiae | Â | Â |
BY4741 | MATa his3−0 leu2−0 met15−0 ura3−0 | Reference [17] |
PRT238 | Wild type | Offered by Dr. Peter Orlean |
Classic Distiller’s Turbo Yeast | Wild type | Reference [18] |
E. coli | Â | Â |
DH5− | General cloning host | Takara |
BW25141 | Cloning host | Provided by Professor William Metcalf |
Plasmids | Â | Â |
pPK2 | ColE1 ORI, Ampr, C. parapsilosis ARS7 ORI, CpURA3 | Reference [19] |
pPICZ−A | With gene ble, encoding the zeocin resistance gene, under the control of Saccharomyces cerevisiae TEF1 promoter. | Life Technologies |
pPK2-zeocin | Derived from pPK2, with zeocin expression cassette inserted at the region of ura3 expression cassette | This study |
pXZ1 | Derived from pPK2, with C. parapsilosis ARS7 ORI deleted. | This study |
pXZ2 | Plasmid which can be maintained in SD108 | This study |
pFRD | With codon optimized frd gene for S. cerevisiae (Genbank accession number AAN40014) | Synthesized by DNA 2.0 |
pRS415 | Yeast centromere with LEU2 marker | NEB |
pRS415-A | Derived from pRS415, with ura3 expression cassette added | This study |
pRS415-B | Derived from pRS415, with pyc expression cassette added | This study |
pRS415-C | Derived from pRS415, with mdh and fumr expression cassette added | This study |
pRS415-D | Derived from pRS415, with frd expression cassette added | This study |
pRS415-E | Derived from pRS415-A, with pyc, mdh, fumr and frd expression cassettes added | This study |