Table 1 Strains and plasmids used in this study
Plasmids and strains | Description | Reference |
|---|---|---|
pUC57 VAMT | Codon-optimised VAMT encoding gene; AMP resistance gene | [11] |
pUC57 LK RADH | Codon-optimised LK RADH encoding gene; AMP resistance gene | GenScript, NJ, USA |
YIplac128-HXT7’p-PGKt | Plasmid with HXT7 truncated promoter and PGK terminator; AMP resistance gene; LEU2 gene | [15] |
YIpOB7 | TDH3 promoter, ADH1 terminator, XDH under PGK1 promoter, with PGK1 terminator; URA3 gene; AMP resistance gene | [25] |
pNW2 | VAMT gene under HXT7 truncated promoter, with PGK1 terminator, LEU2 gene; AMP resistance gene | [11] |
pNW4 | VAMT under TDH3 promoter, with ADH1 terminator; LK RADH under PGK1 promoter, with PGK1 terminator; URA3 gene; AMP resistance gene | This study |
pNW10 | VAMT under TDH3 promoter, with ADH1 terminator; URA3 gene; AMP resistance gene | This study |
S. cerevisiae TMB4150 | CEN.PK2-1C MATa ura3-52 MAL2-8 C SUC2, TRP1, LEU2, HIS3 | Jan Knudsen, unpublished |
S. cerevisiae TMB4350 | CEN.PK113-16B containing pNW2, overexpressing VAMT encoding gene | [11] |
S. cerevisiae TMB4351 | TMB4150 containing pNW4, overexpressing VAMT and LK RADH encoding genes, TRP1, LEU2, HIS3 | This study |
S. cerevisiae TMB4365 | TMB4150 containing 2 times more copies of pNW2 than TMB4350, overexpressing VAMT encoding gene, TRP1, HIS3 | This study |
S. cerevisiae TMB4367 | TMB4150 containing pNW10, overexpressing VAMT encoding gene, TRP1, LEU2, HIS3 | This study |
S. cerevisiae TMB4373 | TMB4365 pNW4, overexpressing VAMT and LK RADH encoding genes, TRP1, HIS3 | This study |